Journal: SpringerPlus

Article Title: Optimization of DNA extraction and PCR protocols for phylogenetic analysis in Schinopsis spp. and related Anacardiaceae

doi: 10.1186/s40064-016-2118-4

Figure Lengend Snippet: 0.8 % Agarose gel electrophoresis depicting results of DNA extraction of some accessions of Schinopsis spp. and related species. a DNA extracted with the optimized protocol. Lanes 1 , 2 , 8 , 14 and 15 molecular marker (Lambda Eco RI/ Hin dIII), 3 and 4 S. lorentzii , 5 and 6 S. marginata , 7 Lithraea molleoides , 9 – 13 S. brasiliensis . b DNA extracted with the DNAEasy Plant Mini Kit (Quiagen Inc. Valencia, CA) used as control. 1 Molecular weight marker (100 bp DNA Ladder), 2 – 11 different specimens of S. brasiliensis ( asterisk reference bands of the marker)

Article Snippet: The DNA yield was estimated by spectrophotometric analysis and by comparing the fluorescence intensity of each sample to 100 ng of Lambda ( Eco RI/ Hin dIII) marker (Promega, USA) (Fig. a) or 325 ng of 100 bp DNA Ladder (Promega) (Fig. b) as standard.

Techniques: Agarose Gel Electrophoresis, DNA Extraction, Marker, Control, Molecular Weight

Journal: SpringerPlus

Article Title: Optimization of DNA extraction and PCR protocols for phylogenetic analysis in Schinopsis spp. and related Anacardiaceae

doi: 10.1186/s40064-016-2118-4

Figure Lengend Snippet: 1.5 % Agarose gel electrophoresis of PCR products of several accessions of Schinopsis spp. and related species. a trn L-F amplified in two parts. Lanes 1 , 20 , 27 molecular marker, 2 – 5 S. boqueronensis , 6 – 7 , 9 S. cornuta , 10 S. peruviana , 11 – 14 S. heterophylla , 15 – 18 , 21 S. brasiliensis , 22 – 23 S. marginata , 24 S. lorentzii , 25 – 26 S. brasiliensis , 28 – 29 Astronium urundeuva and Apterokarpos gardneri , and 8 , 19 without sample. b rps 16. Lanes 1 , 13 and 21 molecular marker, 2 S. peruviana , 3 – 6 S. heterophylla , 5 – 9 , 14 – 15 S. brasiliensis , 10 – 11 S. marginata , 12 S. lorentzii , 16 – 17 Astronium urundeuva and Apterokarpos gardneri , 18 – 19 S. balansae , 20 S. lorentzii , 22 – 24 S. boqueronensis , 25 – 27 S. cornuta . c ndh F. Lanes 1 , 19 and 29 molecular marker, 11 , 12 , 17 – 19 Astronium urundeuva , Apterokarpos gardneri , Lithraea molleoides , Loxopterygium grisebachii and Schinus areira ; other lanes, Schinopsis spp. Arrows indicate the reference bands of the marker Lambda Eco RI/ Hin dIII (564 bp in a , 947 and 831 in b and 831 and 564 in c ) to estimate the amplicons size

Article Snippet: The DNA yield was estimated by spectrophotometric analysis and by comparing the fluorescence intensity of each sample to 100 ng of Lambda ( Eco RI/ Hin dIII) marker (Promega, USA) (Fig. a) or 325 ng of 100 bp DNA Ladder (Promega) (Fig. b) as standard.

Techniques: Agarose Gel Electrophoresis, Amplification, Marker

Journal: SpringerPlus

Article Title: Optimization of DNA extraction and PCR protocols for phylogenetic analysis in Schinopsis spp. and related Anacardiaceae

doi: 10.1186/s40064-016-2118-4

Figure Lengend Snippet: 1.5 % agarose gel electrophoresis of PCR products of some accessions of Schinopsis spp. and related species. a ETS. Lane 1 molecular marker, 2 – 5 S. boqueronensis , 6 – 8 , S. cornuta , 9 , S. peruviana , 10 – 13 , S. heterophylla , 14 – 18 , 21 , 22 , S. brasiliensis , 19 , 20 , S. marginata , 21 , 27 , 28 S. lorentzii , 23 , 24 , 29 – 31 Astronium urundeuva , Apterokarpos gardneri , Lithraea molleoides , Loxopterygium grisebachii and Schinus areira . b ITS2. Line 1 , 12 , 24 molecular marker, 2 S. cornuta , 3 – 6 S. heterophylla , 7 – 10 , 15 , 16 S. brasiliensis , 11 , 12 S. heterophylla , 13 , 18 , 19 , S. lorentzii , 20 – 22 , Lithraea molleoides , Loxopterygium grisebachii and Schinus areira . Arrows indicate the reference bands of the marker Lambda Eco RI/ Hin dIII (564 bp in a and b ) to estimate the amplicons size

Article Snippet: The DNA yield was estimated by spectrophotometric analysis and by comparing the fluorescence intensity of each sample to 100 ng of Lambda ( Eco RI/ Hin dIII) marker (Promega, USA) (Fig. a) or 325 ng of 100 bp DNA Ladder (Promega) (Fig. b) as standard.

Techniques: Agarose Gel Electrophoresis, Marker